Detection and Serogrouping of Dichelobacter nodosus Infection by Use of Direct PCR from Lesion Swabs To Support Outbreak-Specific Vaccination for Virulent Footrot in Sheep
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چکیده
منابع مشابه
Isolation and Characterisation of Dichelobacter Nodosus from Footrot Infected Sheep in Malaysia
Introduction Dichelobacter nodosus is the causative agent of severe footrot, a contagious debilitating disease which affects ruminants particularly sheep and goats. The disease is characterised by separation of the hom of the hoof from the underlying soft tissues resulting in lameness, loss of body condition and reduced wool production (Rood et al. 1996). The first case of footrot in Malaysia w...
متن کاملUnderstanding the molecular epidemiology of the footrot pathogen Dichelobacter nodosus to support control and eradication programs.
The Gram-negative anaerobe Dichelobacter nodosus is the primary etiologic agent of ovine footrot. Few studies of the genetic diversity and epidemiology of D. nodosus have been done, despite the economic cost and welfare implications of the disease. This study examined a large collection of Australian isolates; 735 isolates from footrot-infected sheep from 247 farms in Western Australia (WA) wer...
متن کاملCharacterisation of Dichelobacter nodosus and detection of Fusobacterium necrophorum and Treponema spp. in sheep with different clinical manifestations of footrot.
The aim of this study was to determine the proportion of Dichelobacter nodosus, Fusobacterium necrophorum and Treponema spp. in sheep with different clinical manifestations of footrot compared to healthy sheep both at flock and individual level. The second aim was to characterise D. nodosus with respect to virulence, presence of intA gene and the serogroups. Swab samples (n=1000) from footrot-a...
متن کاملDichelobacter nodosus and Footrot in Swedish Sheep Increased Knowledge and Improved Laboratory Diagnostics
Ovine footrot is a contagious bacterial disease primarily caused by Dichelobacter nodosus. Footrot affects the feet of sheep and is characterised by two major clinical presentations. The milder form consists of inflammation confined to the interdigital space (interdigital dermatitis or benign footrot) and the more severe form includes underrunning of the hoof horn (underrunning or virulent foot...
متن کاملSimultaneous detection and discrimination of virulent and benign Dichelobacter nodosus in sheep of flocks affected by foot rot and in clinically healthy flocks by competitive real-time PCR.
Ovine foot rot caused by Dichelobacter nodosus is affecting sheep worldwide. The current diagnostic methods are difficult and cumbersome. Here, we present a competitive real-time PCR based on allelic discrimination of the protease genes aprV2 and aprB2. This method allows direct detection and differentiation of virulent and benign D. nodosus from interdigital skin swabs in a single test. Clinic...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2018
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.01730-17